New tricks for KDEL receptors

published_or_final_versio

Distributed simulation and industry: Potentials and pitfalls

We present the views of five researchers and practitioners of distributed simulation. Collectively we attempt to address what the implications of distributed simulation are for industry. It is hoped that the views contained herein, and the presentations made by the panelists at the 2002 Winter Simulation Conference will raise awareness and stimulate further discussion on the application of distributed simulation methods and technology in an area that is yet to benefit from the arguable economic benefits that this technique promises

Polemos, pathemata, and plague: Thucydides’ narrative and the tradition of upheaval

Thucydides’ seemingly unscientific list of disasters that accompanied the Peloponnesian War echoes a traditional strand of Greek thought, which Thucydides evidently took seriously as appropriate to his war. © 2017, Duke University Libraries. All rights reserved

Monitoring of sea-ice-atmosphere interface in the proximity of arctic tidewater glaciers: The contribution of marine robotics

The Svalbard archipelago, with its partially closed waters influenced by both oceanic conditions and large tidal glaciers, represents a prime target for understanding the effects of ongoing climate change on glaciers, oceans, and ecosystems. An understanding of the role played by tidewater glaciers in marine primary production is still affected by a lack of data from close proximity to glacier fronts, to which, for safety reasons, manned surface vessels cannot get too close. In this context, autonomous marine vehicles can play a key role in collecting high quality data in dangerous interface areas. In particular, the contribution given by light, portable, and modular marine robots is discussed in this paper. The state-of-the-art of technology and of operating procedures is established on the basis of the experience gained in campaigns carried out by Italian National Research Council (CNR) robotic researchers in Ny-Alesund, Svalbard Islands, in 2015, 2017, and 2018 respectively. The aim was to demonstrate the capability of an Unmanned Semi-Submersible Vehicle (USSV): (i) To collect water samples in contact with the front of a tidewater glacier; (ii) to work in cooperation with Unmanned Aerial Vehicles (UAV) for sea surface and air column characterisation in the proximity of the fronts of the glaciers; and (iii) to perform, when equipped with suitable tools and instruments, repetitive sampling of water surface as well as profiling the parameters of the water and air column close to the fronts of the tidewater glaciers. The article also reports the issues encountered in navigating in the middle of bergy bits and growlers as well as the problems faced in using some sensors at high latitudes

Class II ADP-ribosylation factors are required for efficient secretion of Dengue viruses

This article is available open access through the publisher’s website.Identification and characterization of virus-host interactions are very important steps toward a better understanding of the molecular mechanisms responsible for disease progression and pathogenesis. To date, very few cellular factors involved in the life cycle of flaviviruses, which are important human pathogens, have been described. In this study, we demonstrate a crucial role for class II Arf proteins (Arf4 and Arf5) in the dengue flavivirus life cycle. We show that simultaneous depletion of Arf4 and Arf5 blocks recombinant subviral particle secretion for all four dengue serotypes. Immunostaining analysis suggests that class II Arf proteins are required at an early pre-Golgi step for dengue virus secretion. Using a horseradish peroxidase protein fused to a signal peptide, we show that class II Arfs act specifically on dengue virus secretion without altering the secretion of proteins through the constitutive secretory pathway. Co-immunoprecipitation data demonstrate that the dengue prM glycoprotein interacts with class II Arf proteins but not through its C-terminal VXPX motif. Finally, experiments performed with replication-competent dengue and yellow fever viruses demonstrate that the depletion of class II Arfs inhibits virus secretion, thus confirming their implication in the virus life cycle, although data obtained with West Nile virus pointed out the differences in virus-host interactions among flaviviruses. Our findings shed new light on a molecular mechanism used by dengue viruses during the late stages of the life cycle and demonstrate a novel function for class II Arf proteins.Research Fund for Control of Infectious Diseases of Hong Kong and BNP Paribas Corporate and Investment Banking

The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles

Copyright @ 2008 American Society for Microbiology.The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.The University of Hong Kong and the French Ministry of Health

Definition of the cellular interactome of the highly pathogenic avian influenza H5N1 virus: identification of human cellular regulators of viral entry, assembly, and egress.

This study was supported by the Research Fund for the Control of Infectious Diseases, Food and Health Bureau, Hong Kong SAR Government (#09080892), University Grants Committee Area of Excellence Scheme (AoE/M-12/-06), French Ministry of Health, RESPARI Pasteur network, and the Li Ka Shing Foundation